Season 1 - Episode Guide

Spider-Man discovers that his science teacher, Dr. Curt Connors, has been turned into a deranged lizard due to an experiment gone wrong, and is abducting people throughout Manhattan. 

Norman Osborn hires Spencer Smythe to capture Spider-Man. Smythe creates his deadliest weapon, the Spider Slayer, to accomplish this. Smythe seemingly dies at the end of this episode in an explosion.

The Kingpin offers to provide Alistair Smythe with the funds he requires to build more of Spencer's Spider-Slayer robots, claiming that Spider-Man is responsible for the death of his father. Smythe fails in his bid to kill Spider-Man and the others he bids responsible for his father's death, and as a consequence, the Kingpin makes him work for him until he does kill Spider-Man.

When Peter arrives for a date with Felicia Hardy, she is kidnapped by corrupt scientist Doctor Octopus, in order to force her mother to provide him with ransom money in which she promised to fund his experiments. Complicating things, Peter recognizes the madman as his favorite science mentor from his childhood days.

J. Jonah Jameson and many others believe that Spider-Man is responsible for robberies that have been happening recently. Peter thus decides to search for clues to prove that these robberies were caused by a Spider-Man imposter. He teams up with Detective Lt. Terry Lee in search for clues and confronts Mysterio, a self proclaimed master of the mystic arts.

Mac Gargan, a small-time crook hired by J. Jonah Jameson to follow Peter Parker and find out how he's able to get pictures of Spider-Man, strikes a deal with Jameson to eliminate Spider-Man. Gargan is taken to the lab of Dr. Farley Stillwell, where he is transformed into the supervillain Scorpion.

Peter Parker has an interview with a scientist named Mariah Crawford. When Peter gets to her office she is under attack by the villain Kraven the Hunter after Spider-Man is able to elude him Dr. Crawford explains that Kraven and her used to be in love but when he was seriously injured by some lions she had no choice but to give him this wonder drug, left a former associate of her that can heal all wounds.

John Jameson and fellow astronaut Paul Stevens discover a rock known as "Promethium X", which is said to be more powerful than plutonium. Unknown to the astronaut though, "Promethium X" also contains a mysterious element which manages to break free and tries to consume the two astronauts. The space shuttle crashes on the George Washington Bridge where Rhino is sent by The Kingpin to steal the "Promethium X". Spider-Man arrives on the scene and manages to save Jameson and his fellow astronaut, yet unknown to Spider-Man, Eddie Brock is on the bridge too, and takes photos of him (and Rhino as well). However, when Jonah arrives on the scene, Eddie uses propaganda and frames Spider-Man for stealing something from the shuttle, which leads to a $1 Million dollar bounty on Spider-Man's head. However, Eddie fails to mention to have seen Rhino at the scene also.

Spider-Man focuses his attention on Eddie Brock and Jonah Jameson and warns him to call off his reward, also mentioning Rhino and how Brock neglected to mention him. After Jonah visits his hospitalized son, Jameson finds out from him that Brock was lying and fires him. He also calls off the manhunt for Spider-Man. Noticing another disturbing change in his behavior, Spider-Man seeks the assistance of Doctor Curt Connors, who studies the suit and realizes that it is a Symbiote. Spider-Man then uses the Symbiote's powers to find a clue in Eddie's apartment, only to run into the Shocker, sent by The Kingpin to destroy the evidence Brock has of the crash site. Following Shocker leads Spider-Man to Alistair Smythe and the "Promethium X", which Spider-Man steals. Kingpin and Shocker form a plan to kidnap John Jameson in an attempt to gain the "Promethium X" back.

Spider-Man, returned to his old costume, soon comes across The Rhino and The Shocker. While Spider-Man is able to easily defeat The Rhino, The Shocker manages to get the upper hand. However, an unknown 'ally' arrives and defeats the two villains himself. The new 'ally' proves to be Eddie Brock, who reveals himself as 'Venom'.

Norman Osborn hires the Hobgoblin to assassinate Wilson Fisk, also known as The Kingpin, because of the crime lord's continuous threats against him. Peter Parker saves Fisk just as the Hobgoblin attempts an attack on him. After eluding Spider-Man, the Hobgoblin is fired by Norman as of his unsuccessful assassination on The Kingpin.

Hobgoblin defeats The Kingpin and declares himself the new Kingpin of Crime. Fisk, having managed to escape the Goblin, asks Norman Osborn to assist him in reclaiming his position.

The Chameleon, an international spy, attempts assassination on two diplomats in order to derail the signing of a peace proposal. J. Jonah Jameson assists S.H.I.E.L.D. in an effort to prevent the Chameleon's crime.